伯樂(lè)Sub-Cell GT 水平電泳槽系列
【介紹】
Bio-Rad 提供完整的、應(yīng)用于多種研究的水平電泳設(shè)備系列。一系列廣泛的瓊脂糖電泳槽家族為多種核酸分析技術(shù)提供了解決方案。
水平凝膠在核酸分析方面有許多優(yōu)勢(shì),始終是主要的分子生物學(xué)研究工具。對(duì)于核酸電泳來(lái)說(shuō),水平電泳槽及相應(yīng)系統(tǒng)比垂直系統(tǒng)更經(jīng)濟(jì)、使用更方便。Sub-Cell 浸沒式水平電泳槽(subcell)家族有7種型號(hào),可適合多種應(yīng)用。其中的5 種型號(hào)含有制膠器,可自己鋪制瓊脂糖電泳。如果需要更多的方便,ReadyAgarose 預(yù)制膠可與Mini-Sub 和ReadySub-Cell 兩種型號(hào)匹配,為你節(jié)省了時(shí)間并保證了最高的凝膠可重復(fù)性。
Sub-Cell 家族的主要特點(diǎn):
★ 帶有熒光標(biāo)尺的紫外透光凝膠盤
★ 多種不同大小的手鋪膠選擇
★ 適合所有需要的電泳梳— 多通道移液器兼容電泳梳、高度固定的drop-in電泳梳、高度可調(diào)節(jié)的電泳梳和制備型電泳梳
★ 更換簡(jiǎn)便的電極夾
同時(shí),高通量的96 型和192 型Sub-Cell 電泳槽具有以下特點(diǎn):
★ 緩沖液再循環(huán)口適合高電壓或延長(zhǎng)運(yùn)行的需要
★ 每個(gè)電泳梳可容納48 個(gè)樣品及3 個(gè)標(biāo)準(zhǔn)泳道,孔間距與多通道移液器匹配
★ 凝膠盤寬25 cm,長(zhǎng)度有10-25 cm 多種選擇
★ IEC 1010 ( EN 61010) 電器安全性認(rèn)證
DNA 限制性酶消化樣品在寬式Mini-Sub Cell GT 電泳槽內(nèi)電泳。
Sub-Cell GT 電泳槽
Sub-Cell GT 電泳槽是Sub-Cell 家族中功能最靈活的水平電泳槽,具有最廣范的凝膠長(zhǎng)度、樣品梳和分離 模式選擇,可理想地用于Southern 和northern 雜交等操作。
快速篩選小型電泳槽
如果您需要快速分離DNA,小型水平電泳槽能為你提供速度、簡(jiǎn)便及經(jīng)濟(jì)的解決。Bio-Rad 小型電泳槽 60V、1.5 小時(shí)就能分離EcoRI 或HindIII 消化的lambda噬菌體DNA 產(chǎn)物。小片段DNA 分離只需150V、15 分 鐘。對(duì)于多樣品快速篩選應(yīng)用,寬式subcell 更理想。由于所有小型subcell 都能容納ReadyAgarose 預(yù)制膠,它們也為常規(guī)分離節(jié)省了時(shí)間。
高通量電泳槽
96 型、192 型Sub-Cell 電泳槽是專為多樣品、高通量分析而開發(fā)的。電泳槽和分析電泳梳的設(shè)計(jì)以多通 道移液器的固定間距為基礎(chǔ)。在96 型上應(yīng)用51- 孔電泳梳,一排可電泳48 個(gè)樣品加上3 個(gè)DNA 分子量標(biāo)準(zhǔn) 品。如果使用2 個(gè)電泳梳,微孔板的所有96 個(gè)樣品都可同時(shí)在一塊凝膠上電泳,使其成為PCR 片段分析的 最佳選擇。192 型電泳槽可容納超過(guò)2 個(gè)微孔板的樣品。它的超長(zhǎng)設(shè)計(jì)使它成為RFLP、Southern 和Norhtern 雜 交、分離質(zhì)粒DNA 限制性消化產(chǎn)物的理想選擇。兩種電泳槽都含有許多互換組件。
預(yù)制方便:ReadyAgarose系統(tǒng)
Sub-Cell 產(chǎn)品系列包括ReadySub-Cell 電泳槽,它是ReadyAgarose 預(yù)制膠系統(tǒng)的組成。ReadyAgarose 預(yù)制膠安全地固定于ReadySub-Cell 電泳槽,并具有方便和可重復(fù)性的特點(diǎn)。
ReadyAgarose 預(yù)制膠系統(tǒng)。圖中所示2 種ReadySub-Cell 電泳槽和2 種大小的ReadyAgarose 預(yù)制膠。
Sub-Cell 選擇指南
Mini-Sub Cell GT* | 小型 ReadySub-Cell GT* | 寬式Mini-Sub Cell GT** | 寬式小型 ReadySub-Cell GT** | Sub-Cell GT** | 96型*** Sub-Cell | 192 型***Sub-Cell | |
凝膠盤大小(OD), | 7 x 7 cm | 7 x 10 cm | 15 x 7 cm | 15 x 10 cm | 15 x 10 cm | 25 x 10 cm | 25 x 10 cm |
容納ReadyAgarose | 是 | 是 | 是 | 是 | 否 | 否 | 否 |
包括制膠器 | 有 | 無(wú) | 有 | 無(wú) | 有 | 有 | 有 |
樣品通量 | 8–30? | 8 或12 | 10–60? | 20, 32, | 1–120?? | 24–96? | 24–192?? |
基座緩沖液容量 | ~270 ml | ~270 ml | ~650 ml | ~650 ml | ~1 L | ~2 L | ~3 L |
緩沖液再循環(huán) | 無(wú) | 無(wú) | 無(wú) | 無(wú) | 無(wú) | 有 | 有 |
溴酚藍(lán)染料 | 4.5 cm/hr | ~4.5 cm/hr | ~4.5 cm/hr | ~3.0 cm/hr | ~6.2 cm/hr | ~5.2 cm/hr | |
遷移速度 | (75 V) | (75 V) | (75 V) | (75 V) | (200 V) | (200 V) |
** 電泳梳架(貨號(hào)#170-4320)和高度可調(diào)電泳梳適合所有型號(hào)。
***電泳梳架(貨號(hào)#170-4525)和高度可調(diào)電泳梳適合兩種型號(hào)。
? 樣品通量定義:每塊凝膠1-2 個(gè)電泳梳的通量值。
?? 樣品通量定義:每塊凝膠1-4 個(gè)電泳梳的通量值。
多種凝膠大小的手鋪選擇
Sub-Cell 電泳槽含有兩種用于手工鋪膠的配件。使用相應(yīng)大小的凝膠盤,鋪制門(圖右上角)可方便地在凝膠槽里 直接鋪制。制膠器(圖右下角)提供無(wú)膠帶鋪制,并增加了鋪制多種尺寸凝膠的靈活性。
制膠指南
電泳槽 | 凝膠盤規(guī)格 | 鋪制門或制膠器 | 僅制膠器 |
Mini-Sub Cell GT | 7 x 7 cm | ★ | |
7 x 10 cm | ★ | ||
寬式Mini-Sub Cell GT | 15 x 7 cm | ★ | |
15 x 10 cm | ★ | ||
Sub-Cell GT | 15 x 10 cm | ★ | |
15 x 15 cm | ★ | ||
15 x 20 cm | ★ | ||
15 x 25 cm | ★ |
Sub-Cell GT 與原始Sub-Cell 系統(tǒng)(1996 年以前售出的)的兼容性
除了2 個(gè)配件外,所有Sub-Cell GT 配件都可與原始Sub-Cell 系統(tǒng)兼容。新的Mini-Sub Cell GT 電泳槽的凝膠 盤更深,能鋪制更厚的凝膠,因此不能與原始Mini-Sub 凝膠盤互換。同時(shí),高度固定的drop-in 電泳梳適合于Sub Cell GT 的UV- 透光盤,因?yàn)檫@些盤才有放置電泳梳的凹口。仍然提供原始Mini-Sub Cell 凝膠盤。所有GT 電泳槽 凝膠盤都適合原始寬式Mini-Sub Cell 和Sub-Cell 系統(tǒng)。提供原始Sub-Cell 和Sub-Cell GT 系統(tǒng)的備用部件。詳細(xì)信 息,請(qǐng)與當(dāng)?shù)?span>Bio-Rad 銷售代表聯(lián)系。
【訂貨信息】
目錄 | 描述 |
Sub-Cell GT Systems | |
170-4401 | Sub-Cell GT System, with 15 x 10 cm tray |
170-4402 | Sub-Cell GT System, with 15 x 15 cm tray |
170-4403 | Sub-Cell GT System, with 15 x 20 cm tray |
170-4404 | Sub-Cell GT System, with 15 x 25 cm tray |
170-4405 | Wide Mini-Sub Cell GT System, with 15 x 7 cm tray |
170-4406 | Mini-Sub Cell GT System, with 7 x 7 cm tray |
170-4466 | Mini-Sub Cell GT System, with 7 x 10 cm tray |
170-4467 | Mini-Sub Cell GT System, with 7 x 10 cm tray, mini-gel caster |
170-4468 | Wide Mini-Sub Cell GT System, with 15 x 10 cm tray |
170-4469 | Wide Mini-Sub Cell GT System, with 15 x 10 cm tray, mini-gel caster |
170-4481 | Sub-Cell GT System, with 15 x 10 cm tray, gel caster |
170-4482 | Sub-Cell GT System, with 15 x 15 cm tray, gel caster |
170-4483 | Sub-Cell GT System, with 15 x 20 cm tray, gel caster |
170-4484 | Sub-Cell GT System, with 15 x 25 cm tray, gel caster |
170-4485 | Wide Mini-Sub Cell GT System, with 15 x 7 cm tray, mini-gel caster |
170-4486 | Mini-Sub Cell GT System, with 7 x 7 cm tray, mini-gel caster |
Sub-Cell Models 96 and 192 Systems | |
170-4500 | Sub-Cell Model 96 Cell, with 25 x 10 cm tray, gel caster |
170-4501 | Sub-Cell Model 96 Cell, with 25 x 15 cm tray, gel caster |
170-4502 | Sub-Cell Model 96 Cell, with 25 x 10 cm tray |
170-4503 | Sub-Cell Model 96 Cell, with 25 x 15 cm tray |
170-4504 | Sub-Cell Model 192 Cell, with 25 x 10 cm tray, gel caster |
170-4505 | Sub-Cell Model 192 Cell, with 25 x 15 cm tray, gel caster |
170-4506 | Sub-Cell Model 192 Cell, with 25 x 20 cm tray, gel caster |
170-4507 | Sub-Cell Model 192 Cell, with 25 x 25 cm tray, gel caster |
170-4508 | Sub-Cell Model 192 Cell, with 25 x 10 cm tray |
170-4509 | Sub-Cell Model 192 Cell, with 25 x 15 cm tray |
170-4510 | Sub-Cell Model 192 Cell, with 25 x 20 cm tray |
170-4511 | Sub-Cell Model 192 Cell, with 25 x 25 cm tray |
Combs for Mini Sub-Cell Electrophoresis Systems | |
170-4332 | 15-Well Comb, 2.6 mm wide wells, 1.0 mm thick, adjustable height |
170-4333 | 8-Well Comb, 5.5 mm wide wells, 1.0 mm thick, adjustable height |
170-4342 | Preparative Comb, 1 preparative, 2 reference wells, 3 mm thick, adjustable height |
170-4460 | Preparative Comb, 1 preparative, 2 reference wells, 1.5 mm thick, fixed height |
170-4461 | Preparative Comb, 2 preparative, 2 reference wells, 1.5 mm thick, fixed height |
170-4462 | 8-Well Comb, 5.5 mm wide wells, 0.75 mm thick, fixed height |
170-4463 | 8-Well Comb, 5.5 mm wide wells, 1.5 mm thick, fixed height |
170-4464 | 15-Well Comb, 3 mm wide wells, 0.75 mm thick, fixed height |
170-4465 | 15-Well Comb, 3 mm wide wells, 1.5 mm thick, fixed height |
Combs for Sub-Cell and Wide Mini-Sub Cell Electrophoresis Cells | |
170-4321 | 20-Well Comb, 14 cm wide, 0.75 mm thick, adjustable height |
170-4322 | 20-Well Comb, 14 cm wide, 1.5 mm thick, adjustable height |
170-4323 | 15-Well Comb, 14 cm wide, 0.75 thick, adjustable height |
170-4324 | 15-Well Comb, 14 cm wide, 1.5 mm thick, adjustable height |
170-4325 | 10-Well Comb, 14 cm wide, 0.75 mm thick, adjustable height |
170-4326 | 10-Well Comb, 14 cm wide, 1.5 mm thick, adjustable height |
170-4344 | 30-Well Comb, 14 cm wide, 1.5 mm thick, adjustable height |
170-4443 | 10-Well Comb, 14 cm wide, 0.75 mm thick, fixed height |
170-4444 | 10-Well Comb, 14 cm wide, 1.5 mm thick, fixed height |
170-4445 | 15-Well Comb, 14 cm wide, 0.75 mm thick, fixed height |
170-4446 | 15-Well Comb, 14 cm wide, 1.5 mm thick, fixed height |
170-4448 | 20-Well Comb, 14 cm wide, 1.5 mm thick, fixed height |
170-4449 | 30-Well Comb, 14 cm wide, 1.5 mm thick, fixed height |
Multichannel Pipet-Compatible Combs for Sub-Cell GT and Wide Mini-Sub Cell GT | |
170-4450 | Multichannel Pipet-Compatible Comb, 10 wells, 0.75 mm thick, fixed height |
170-4451 | Multichannel Pipet-Compatible Comb, 10 wells, 1.5 mm thick, fixed height |
170-4452 | Multichannel Pipet-Compatible Comb, 14 wells, 0.75 mm thick, fixed height |
170-4453 | Multichannel Pipet-Compatible Comb, 14 wells, 1.5 mm thick, fixed height |
170-4454 | Multichannel Pipet-Compatible Comb, 18 wells, 0.75 mm thick, fixed height |
170-4455 | Multichannel Pipet-Compatible Comb, 18 wells, 1.5 mm thick, fixed height |
170-4456 | Multichannel Pipet-Compatible Comb, 26 wells, 0.75 mm thick, fixed height |
170-4457 | Multichannel Pipet-Compatible Comb, 26 wells, 1.5 mm thick, fixed height |
Preparative Combs for Sub-Cell and Wide Mini-Sub Cell Systems | |
170-4328 | Preparative Comb for Sub-Cell and Wide Mini-Sub Cell Systems, 1 preparative, 2 reference wells, 3 mm thick, adjustable height |
170-4440 | Preparative Comb for Sub-Cell and Wide Mini-Sub Cell Systems, 1 preparative, 2 reference wells, 1.5 mm thick, fixed height |
170-4441 | Preparative Comb for Sub-Cell and Wide Mini-Sub Cell Systems, 2 preparative, 2 reference wells, 1.5 mm thick, adjustable height |
Adjustable-Height Combs for Sub-Cell Systems 96 and 192 | |
170-4527 | 26-Well Comb, 6 mm wide wells, 1.5 mm thick |
170-4529 | 51-Well Comb, 3 mm wide wells, 1.5 mm thick |
170-4530 | Preparative Comb, 2 or 4 preparative, 2 reference wells, 0.75 mm thick |
170-4531 | Preparative Comb, 2 or 4 preparative, 2 reference wells, 1.5 mm thick |
Comb Holders | |
170-4320 | Comb Holder, for Sub-Cell and wide Mini-Sub Cell adjustable-height combs |
170-4331 | Mini-Comb Holder, for Mini-Sub Cell adjustable-height combs |
170-4525 | Sub-Cell Models 96 and 192 Comb Holder |